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EZorb CalciumĀ®   Calcium Aspartate Anhydrous

Absorption Test


January 12, 2000
Tang, J. F.

Absorption Test of Calcium Aspartate Anhydrous

Various calcium compounds including calcium aspartate anhydrous, calcium citrate,
calcium aspartate dihydrate, calcium amino acid chelates and calcium carbonate, were
tested for calcium absorption rate. It is demonstrated that calcium aspartate anhydrous
has a significantly higher calcium absorption rate than those of other calcium compounds
tested.


Materials and Equipment

(1) Rats: pure breed SD rats of average body weight of 135g (120-150g) were provided
by Academic Sinica.
(2) Apparatus: VM3 Vortex Mixer was produced by Shanghai Guanghua Scientific
Apparatus and Materials Co. Ltd; Centrifuge CR7 was produced by Hitachi; DT-100
Single Pan Balance was produced by Beijing Optical Apparatus Factory.
(3) Calcium deficient forage.
(4) Calcium aspartate anhydrous (13%) was formulated into solutions with calcium
concentration of 3.6mg/ml.
(5) Calcium citrate (20.3%) was formulated into a mixed suspension with calcium
concentration of 3.6mg/ml.
(6) Calcium aspartate dihydrate (19.2%) was formulated into a mixed suspension with
3.6mg/ml calcium concentration.
(7) Calcium amino acid chelates (11.2%), was formulated into a mixed suspension with
3.6mg/ml calcium concentration.
(8) Calcium carbonate (40.0%) was formulated into a mixed suspension, which has a
calcium concentration of 3.6mg/ml.


Groups and Dosage

(1) Group A: each rat was dosed orally (gavage) twice (1ml each time) a day with
53mg/kg body weight of calcium aspartate anhydrous.
(2) Group B: each rat was administrated orally twice (1ml each time) a day with 53mg/kg
body weight of calcium citrate.
(3) Group C: each rat was fed twice (1ml each time) a day with 53mg/kg body weight of
calcium aspartate dihydrate.
(4) Group D: each rat was dosed twice (1ml each time) a day with 53mg/kg body weight
of calcium amino acid chelates.

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January 12, 2000
Tang, J. F.

(5) Group E: each rat was fed twice (1mg each time) a day with 53mg/kg body weight of
calcium carbonate.


Procedures

(1) One hundred twenty pure breed SD rats were divided evenly into six groups set forth:
the calcium aspartate anhydrous group, the calcium citrate group, the calcium amino
acid chelates group and the calcium carbonate group.
(2) Rats in every group were fed with calcium deficient forage for three days before
administration of prepared agents.
(3) Rats were contained separately in individual stainless steel metabolic cages. Excreta
were collected for seven consecutive days. The excreta were then nitrated and
assayed for calcium using AAS method.
(4) At the end of testing 5-6ml plasma was drawn from the heart and centrifuged. Serum
was isolated and assayed for calcium using AAS method.


Results

(1) Urine calcium concentration is reported in Table 1.

Table 1. Urine Calcium Concetration
Urine Calcium Concetration

(2) Stool calcium concentration and calcium absorption rates are shown in Table 2 and
the chart below.

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January 12, 2000
Tang, J. F.
Table II. Stool Calcium Concentration
Urine Calcium Concetration

* Group A: calcium aspartate anhydrous B: calcium citrate C: calcium aspartate dihydrate
D: calcium amino acid chelates E: calcium carbonate

Figure 1. Calcium Absorption Test Results

Urine Calcium Concetration

(2) Serum calcium level
Serum Ca levels after administration of different calcium preparations are shown in Table
III.

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January 12, 2000
Tang, J. F.
Table III. Serum Ca Levels After Administration
Urine Calcium Concetration

Conclusion

The test results show that calcium aspartate anhydrous is well absorbed in rat body and
has a significantly higher calcium absorption rate than those of other calcium agents
including calcium citrate, hydrous form of calcium aspartate, calcium amino acid chelates
and calcium carbonate.

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